Addgene - pGL3Basic-Mdm3-T5 Plasmid Data

Plasmid 32373: pGL3Basic-Mdm3-T5

Gene/insert name:
Mdm2 promoter
Insert size:
144
Species:
M. musculus (mouse)
Entrez Gene:
Mdm2 (1700007J15Rik, AA415488, Mdm-2)
Vector backbone:
pGL3Basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4818
Cloning method:
TOPO Cloning
5' sequencing primer:
CTAGCAAAATAGGCTGTCCC List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Hong Wu
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PTEN regulates Mdm2 expression through the P1 promoter. Chang et al (J Biol Chem. 2004 Jul 9;279(28):29841-8. Epub 2004 Apr 16. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32373" in your Materials and Methods section.