Vector typebacterial integration vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameint Ms6; gfpm2+; xylEm; ttsbiA, ttsbiB
- Cloning method Restriction Enzyme
- 5′ cloning site Hpa I (not destroyed)
- 3′ cloning site Kpn I (not destroyed)
- 5′ sequencing primer pALEX_seq2 (Common Sequencing Primers)
Terms and Licenses
This is an improved Ms6 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE expression as reporter genes; terminators to protect expression cassette against transcriptional interference.
Please note that the conserved N->S point mutation found in the Addgene QC sequence of the Ms6 integrase gene is of no consequence and the plasmid is still fully functional for integration.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pML1361 was a gift from Michael Niederweis (Addgene plasmid # 32379)
For your References section:Taking phage integration to the next level as a genetic tool for mycobacteria. Huff J, Czyz A, Landick R, Niederweis M. Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6. 10.1016/j.gene.2010.07.012 PubMed 20692326
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.