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pML1361
(Plasmid #32379)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    int Ms6; gfpm2+; xylEm; ttsbiA, ttsbiB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hpa I (not destroyed)
  • 3′ cloning site Kpn I (not destroyed)
  • 5′ sequencing primer pALEX_seq2
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This is an improved Ms6 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE expression as reporter genes; terminators to protect expression cassette against transcriptional interference.

Please note that the conserved N->S point mutation found in the Addgene QC sequence of the Ms6 integrase gene is of no consequence and the plasmid is still fully functional for integration.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML1361 was a gift from Michael Niederweis (Addgene plasmid # 32379)
  • For your References section:

    Taking phage integration to the next level as a genetic tool for mycobacteria. Huff J, Czyz A, Landick R, Niederweis M. Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6. 10.1016/j.gene.2010.07.012 PubMed 20692326