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Browse > Michael Niederweis > Huff et al > pML1361
Plasmid 32379: pML1361

  • int Ms6; gfpm2+; xylEm; ttsbiA, ttsbiB

  • pMl603
    (Search Vector Database)

  • bacterial integration vector

  • Hpa I

  • No

  • Kpn I

  • No

  • pALEX_seq2 List of Sequencing Primers

  • Hygromycin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (4)

  • View map

  • Michael Niederweis

  • MTA

Comments: 

This is an improved Ms6 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE expression as reporter genes; terminators to protect expression cassette against transcriptional interference.

Please note that the conserved N->S point mutation found in the Addgene QC sequence of the Ms6 integrase gene is of no consequence and the plasmid is still fully functional for integration.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Taking phage integration to the next level as a genetic tool for mycobacteria. Huff et al (Gene. 2010 Nov 15;468(1-2):8-19. Epub 2010 Aug 6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32379" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only