Addgene - Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D Plasmid Data

Plasmid 32460: Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D

Gene/insert name:
Calcium/Calmodulin dependent protein kinase kinase beta
Alt name:
CaMKKbeta
Alt name:
CaMKK2
Insert size:
1600
Species:
M. musculus (mouse)
GenBank ID:
NM_145358.1
Entrez Gene:
Camkk2 (6330570N16Rik, AW061083, mKIAA0787)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Mutation:
S129D, S133D, S137D
Vector backbone:
pRRLsin.PPT.hPGK.FLAG-CaMKKbeta-IRES.GFP
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
8000
Promoter:
human PGK
Cloning site 5':
NheI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
Insert specific List of Sequencing Primers
3' sequencing primer:
Insert specific
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Anthony Means
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Ca2+/Calmodulin-dependent Protein Kinase Kinase {beta} Is Regulated by Multisite Phosphorylation. Green et al (J Biol Chem. 2011 Aug 12;286(32):28066-79. Epub 2011 Jun 13. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32460" in your Materials and Methods section.

Price: $65.00

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