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Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D
(Plasmid #32460)

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Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone size w/o insert (bp) 8000
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.


  • Gene/Insert name
    Calcium/Calmodulin dependent protein kinase kinase beta
  • Alt name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    S129D, S133D, S137D
  • GenBank ID
  • Entrez Gene
    Camkk2 (a.k.a. 6330570N16Rik, AW061083, mKIAA0787)
  • Promoter human PGK
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Insert specific
  • 3′ sequencing primer Insert specific
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D was a gift from Anthony Means (Addgene plasmid # 32460)
  • For your References section:

    Ca2+/Calmodulin-dependent Protein Kinase Kinase {beta} Is Regulated by Multisite Phosphorylation. Green MF, Scott JW, Steel R, Oakhill JS, Kemp BE, Means AR. J Biol Chem. 2011 Aug 12;286(32):28066-79. Epub 2011 Jun 13. 10.1074/jbc.M111.251504 PubMed 21669867