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Plasmid 32520: pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W
  • Constitutively active Rheb (S16H)

  • R. norvegicus (rat)

  • Rheb ()

  • S16H to make constitutively active

  • pHAGE-CMV-Rheb-IRES-eGFP-W
    (Search Vector Database)

  • Addgene plasmid #32519

  • Mammalian Expression, Lentiviral

  • CMV

  • NotI

  • No

  • BamHI

  • No

  • CMV forward List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • View sequences (2)
  • Mustafa Sahin

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

The constitutively activating S16H mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W (Addgene plasmid #32519) by site directed mutagenesis.

The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Tsc2-Rheb signaling regulates EphA-mediated axon guidance. Nie et al (Nat Neurosci. 2010 Feb;13(2):163-72. Epub 2010 Jan 10. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32520" in your Materials and Methods section.