|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32580||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDONR221 P1-P4
Vector typeGateway cloning vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEnhanced Synapsin Promoter
SpeciesH. sapiens (human)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bySYN and ESYN sequences used in these plasmids were provided by Hiroyuki Hioki from the Graduate School of Medicine, Kyoto University, Japan
Terms and Licenses
- Not Available to Industry
The attL1 site within this plasmid is missing the last 3 nucleotides. The depositor states that this discrepancy does not affect plasmid function.
Gene Therapy 14, 872-882 (June 2007) doi:10.1038/sj.gt.3302924
Efficient gene transduction of neurons by lentivirus with enhanced neuron-specific promoters
H Hioki, H Kameda, H Nakamura, T Okunomiya, K Ohira, K Nakamura, M Kuroda, T Furuta and T Kaneko
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR-L1-ESYN-L4 was a gift from Matthew Nolan (Addgene plasmid # 32580 ; http://n2t.net/addgene:32580 ; RRID:Addgene_32580)
For your References section:A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or Down-Regulate Neuronal Gene Expression in vivo. White MD, Milne RV, Nolan MF. Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4. 10.3389/fnmol.2011.00008 PubMed 21772812