|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32846||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid 20071
- Backbone size w/o insert (bp) 4885
Growth in Bacteria
Bacterial Resistance(s)Amp 20ug/ml
Growth instructions2YT media for 20hours
Copy numberLow Copy
Insert Size (bp)1077
- Promoter CAGG
/ Fusion Protein
- tdTomato (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pACAGW-oChiEFtdTomato-AAV was a gift from Rory McQuiston (Addgene plasmid # 32846 ; http://n2t.net/addgene:32846 ; RRID:Addgene_32846)
For your References section:Optogenetic Stimulation of the Corticothalamic Pathway Affects Relay Cells and GABAergic Neurons Differently in the Mouse Visual Thalamus. Chris W. D. Jurgens, Karen A. Bell, A. Rory McQuiston, William Guido.. PLoS ONE 7(9): e45717 10.1371/journal.pone.0045717