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pACAGW-oChiEFtdTomato-AAV
(Plasmid #32846)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32846 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACAGW-ChR2-Venus-AAV
  • Backbone manufacturer
    Addgene plasmid 20071
  • Backbone size w/o insert (bp) 4885
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Amp 20ug/ml
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    2YT media for 20hours
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    oChiEF
  • Species
    Chlamydomonas reinhardtii
  • Insert Size (bp)
    1077
  • Promoter CAGG
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACAGW-oChiEFtdTomato-AAV was a gift from Rory McQuiston (Addgene plasmid # 32846)
  • For your References section:

    Optogenetic Stimulation of the Corticothalamic Pathway Affects Relay Cells and GABAergic Neurons Differently in the Mouse Visual Thalamus. Chris W. D. Jurgens, Karen A. Bell, A. Rory McQuiston, William Guido.. PLoS ONE 7(9): e45717 10.1371/journal.pone.0045717