Addgene - pAC-luc-TimRA Plasmid Data

Plasmid 33133: pAC-luc-TimRA

Gene/insert name:
Tim-RA 3' UTR
Species:
D. melanogaster (fly)
Entrez Gene:
tim (Dmel_CG3234, CG3234, Dmel\CG3234, Ritsu, TIM, Tim, dTIM, dTim, dtim, rit, tim1)
Fusion protein or tag:
Firefly Luciferase
Terminal:
N terminal on backbone
Vector backbone:
pAc5.1/V5-His B
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression, Luciferase
Modifications to Backbone:
pAc-Luciferase was constructed by ligation of the PCR product containing the Renilla Luciferase gene and an artificial poly-linker sequence into the parent vector.
Promoter:
Actin 5C
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
AC5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin, Blasticidin
Sequence: View sequences (2)
Principal Investigator:
Michael Rosbash
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

pAc-Luc-TimRA was constructed by amplifying the genomic region containing the clk 3′ UTR plus 300 bases downstream from the annotated end of the mRNA and ligating the PCR product into pAc-Luciferase.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A role for microRNAs in the Drosophila circadian clock. Kadener et al (Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33133" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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