Addgene - pAC-Per Plasmid Data

Plasmid 33157: pAC-Per

Gene/insert name:
Per
Species:
D. melanogaster (fly)
Entrez Gene:
per (Dmel_CG2647, CG2647, Clk, Dmel\CG2647, EG:155E2.4, PER, Per, clk-6, dPER, dper, unamed)
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Fusion protein or tag:
6xHIS
Terminal:
C terminal on backbone
Vector backbone:
pAc5.1/V5-His B
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression, Retroviral
Backbone size w/o insert (bp):
5400
Promoter:
Actin 5C
Cloning site 5':
none
Site destroyed during cloning:
Unknown
Cloning site 3':
none
Site destroyed during cloning:
Unknown
5' sequencing primer:
AC5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Hygromycin, Blasticidin
Sequence: View sequences (1)
Principal Investigator:
Michael Rosbash
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The doubletime and CKII kinases collaborate to potentiate Drosophila PER transcriptional repressor activity. Nawathean et al (Mol Cell. 2004 Jan 30;13(2):213-23. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33157" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

33153: pAC-dCLK