Addgene - pAC-dCLK Plasmid Data

Plasmid 33153: pAC-dCLK

Gene/insert name:
CLK
Species:
D. melanogaster (fly)
Entrez Gene:
Clk (Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk)
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Fusion protein or tag:
6xHIS
Terminal:
C terminal on backbone
Vector backbone:
pAc5.1/V5-His B
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression, Retroviral
Backbone size w/o insert (bp):
5400
Promoter:
Actin 5C
Cloning site 5':
NotI
Site destroyed during cloning:
Yes
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
AC5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Hygromycin, Blasticidin
Sequence: View sequences (1)
Principal Investigator:
Michael Rosbash
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter. McDonald et al (Mol Cell Biol. 2001 Feb;21(4):1207-17. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33153" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

33157: pAC-Per