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CMV566 empty vector
(Plasmid #33349)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 33349 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRc/CMV
  • Backbone manufacturer
    5500
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with NdeI and HindIII, and the following annealed oligonucleotide was inserted with a hemagglutinin epitope (YPYDVPDYASL) and NdeI-HindIII cloning site was made using the following pair of complementary oligonucleotides: 5′-AGCTCCACCATGGCGTATCCCTACGACGTGCCCGATTATGCCCATATGTGATGAAGCTT-3′ (underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5′-CTAGAAGCTTCATCACATATGGGCATAATCGGGCACGTCGTAGGGATACGCCATGGTGG-3′ (underlined bases are restriction site for XbaI).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV566 empty vector was a gift from Charles Vinson (Addgene plasmid # 33349 ; http://n2t.net/addgene:33349 ; RRID:Addgene_33349)
  • For your References section:

    SREBP-1 dimerization specificity maps to both the helix-loop-helix and leucine zipper domains: use of a dominant negative. Rishi V, Gal J, Krylov D, Fridriksson J, Boysen MS, Mandrup S, Vinson C. J Biol Chem. 2004 Mar 19;279(12):11863-74. Epub 2003 Dec 31. 10.1074/jbc.M308000200 PubMed 14702347