Addgene - CMV500 A-C/EBPβ Plasmid Data

Plasmid 33363: CMV500 A-C/EBPβ

Gene/insert name:
Cebpb
Species:
M. musculus (mouse)
Entrez Gene:
Cebpb (RP24-151H6.1, C/EBPbeta, CRP2, IL-6DBP, LAP, LIP, NF-IL6, NF-M, Nfil6)
Fusion protein or tag:
FLAG
Terminal:
N terminal on insert
Mutation:
Dominant Negative (see comments)
Vector backbone:
CMV500
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Promoter:
CMV
Promoter:
CMV
Cloning site 5':
None
Site destroyed during cloning:
Unknown
Cloning site 3':
None
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-Fwd List of Sequencing Primers
3' sequencing primer:
BGH-Rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Charles Vinson
Terms and Licenses:
MTA

Comments:

This is the dominant negative Cebpb- which contains an N-terminal acidic extension (LEQRAEELARENEELEKEAEELEQENAE) fused to the HLH-ZIP region of Cebpb.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 33363" in your Materials and Methods section.