Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34597||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6470
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2300
Entrez GeneKLF4 (a.k.a. EZF, GKLF)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site PmeI (destroyed during cloning)
- 3′ cloning site PmeI (destroyed during cloning)
- 5′ sequencing primer use KLF4 internal primers
- 3′ sequencing primer use KLF4 internal primers (Common Sequencing Primers)
This is a translation reporter. KLF4 cDNA (the entire CDS and 3' UTR) was released from pCTV3K-SQC7 and cloned into the 3'UTR of the firefly luciferase transgene in pMIR-Report-Luciferase. The insert/cDNA fragment was generated by cleavage at a KLF4-internal Asp718 site within the KLF4 5' UTR, and a downstream SalI site. pCTV3K-SQC7 was described in Foster KW et al., Cell Growth & Differentiation, Vol. 10, 423–434, June 1999.
Please note that there is a G missing in the 3' UTR of KLF4.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIR-Report-Luc-KLF4-FL was a gift from Michael Ruppert (Addgene plasmid # 34597 ; http://n2t.net/addgene:34597 ; RRID:Addgene_34597)
For your References section:A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. 10.1128/MCB.01189-10 PubMed 21518959
Map uploaded by the depositor.