Addgene - phOCT4-Luc Plasmid Data

Plasmid 17221: phOCT4-Luc

Gene/insert name:
Upstream region of human OCT4
Insert size:
5068
Species:
H. sapiens (human)
Entrez Gene:
POU5F1 (DADB-104B20.2, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
Mutation:
None
Vector backbone:
pGV-BM2
(Search Vector Database)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4842
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
BglII
Site destroyed during cloning:
No
5' sequencing primer:
pGV-2U List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi et al (Cell. 2007 Nov 30. 131(5):861-72. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17221" in your Materials and Methods section.