Addgene - Nanog5P reporter Plasmid Data

Plasmid 16337: Nanog5P reporter

Gene/insert name:
Nanog promoter
Insert size:
2500
Species:
M. musculus (mouse)
Entrez Gene:
Nanog (2410002E02Rik, ENK, ecat4)
Fusion protein or tag:
Luciferase
Terminal:
C terminal on backbone
Vector backbone:
pGL3-basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4800
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
RVprimer3 List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Austin Cooney
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

5' Nanog Luciferase reporter, contains 2.5 kb of 5' promoter region of the mouse Nanog gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Orphan nuclear receptor GCNF is required for the repression of pluripotency genes during retinoic acid-induced embryonic stem cell differentiation. Gu et al (Mol Cell Biol. 2005 Oct . 25(19):8507-19. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16337" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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