Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

Nanog5P reporter
(Plasmid #16337)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16337 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nanog promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • Entrez Gene
    Nanog (a.k.a. 2410002E02Rik, ENK, ecat4)
  • Promoter Mouse Nanog promoter
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

5' Nanog Luciferase reporter, contains 2.5 kb of 5' promoter region of the mouse Nanog gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nanog5P reporter was a gift from Austin Cooney (Addgene plasmid # 16337)
  • For your References section:

    Orphan nuclear receptor GCNF is required for the repression of pluripotency genes during retinoic acid-induced embryonic stem cell differentiation. Gu P, LeMenuet D, Chung AC, Mancini M, Wheeler DA, Cooney AJ. Mol Cell Biol. 2005 Oct . 25(19):8507-19. 10.1128/MCB.25.19.8507-8519.2005 PubMed 16166633