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Plasmid 16337: Nanog5P reporter
  • Nanog promoter

  • 2500

  • M. musculus (mouse)

  • Nanog (2410002E02Rik, ENK, ecat4)

  • Luciferase

  • C terminal on backbone

  • pGL3-basic
    (Search Vector Database)

  • Promega

  • Mammalian Expression, Luciferase

  • 4800

  • KpnI

  • No

  • XhoI

  • No

  • RVprimer3 List of Sequencing Primers

  • LucNrev

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Austin Cooney

  • UBMTA
    Luciferase Limited Use Label License

Comments: 

5' Nanog Luciferase reporter, contains 2.5 kb of 5' promoter region of the mouse Nanog gene.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Orphan nuclear receptor GCNF is required for the repression of pluripotency genes during retinoic acid-induced embryonic stem cell differentiation. Gu et al (Mol Cell Biol. 2005 Oct . 25(19):8507-19. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 16337" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only