Addgene - P413TEF-PYK1 Plasmid Data

Plasmid 34605: P413TEF-PYK1

Gene/insert name:
PYK1
Alt name:
YAL038W
Insert size:
1503
Species:
S. cerevisiae (budding yeast)
Vector backbone:
P413TEF
(Search Vector Database)
Backbone manufacturer:
ATCC#87362 (PMID:7737504)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5557
Promoter:
TEF
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pBluescriptSK List of Sequencing Primers
3' sequencing primer:
CYC1
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
HIS3
Sequence: View sequences (2)
Principal Investigator:
Markus Ralser
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Pyruvate kinase triggers a metabolic feedback loop that controls redox metabolism in respiring cells. Gruning et al (Cell Metab. 2011 Sep 7;14(3):415-27. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34605" in your Materials and Methods section.