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LAMP1-mGFP
(Plasmid #34831)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34831 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4729
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAMP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mGFP (C terminal on backbone)
    • Kozak (N terminal on insert)
    • GSTGSTGSTGA linker (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid contains a variant of GFP, where the leucine at position 221 has been replaced by lysine to avoid anti-parallel dimerization.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LAMP1-mGFP was a gift from Esteban Dell'Angelica (Addgene plasmid # 34831 ; http://n2t.net/addgene:34831 ; RRID:Addgene_34831)
  • For your References section:

    Distribution and dynamics of Lamp1-containing endocytic organelles in fibroblasts deficient in BLOC-3. Falcon-Perez JM, Nazarian R, Sabatti C, Dell'Angelica EC. J Cell Sci. 2005 Nov 15;118(Pt 22):5243-55. Epub 2005 Oct 25. 10.1242/jcs.02633 PubMed 16249233