LZRS PU.1 WT
(Plasmid #34835)

• Depositing Lab: Ellen Rothenberg
• Publication: Anderson et al Immunity. 2002 Feb;16(2):285-96.

Backbone

• Vector backbone: LZRSpBMN-linker-IRES-EGFP
• Backbone manufacturer: Hergen Spits and coworkers
• Backbone size w/o insert (bp): 12443
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PU.1
• Species: M. musculus (mouse)
• Insert Size (bp): 839
• Entrez Gene: Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
• Promoter: LTR
• Tags / Fusion Proteins:
  • IRES (C terminal on backbone)
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry