Addgene - LZRS PU.1 WT Plasmid Data

Plasmid 34835: LZRS PU.1 WT

Gene/insert name:
PU.1
Insert size:
839
Species:
M. musculus (mouse)
Entrez Gene:
Sfpi1 (RP23-20F9.1, Dis-1, Dis1, PU.1, Sfpi-1, Spi-1, Spi1, Tcfpu1, Tfpu.1)
Fusion protein or tag:
IRES
Terminal:
C terminal on backbone
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Vector backbone:
LZRSpBMN-linker-IRES-EGFP
(Search Vector Database)
Backbone manufacturer:
Hergen Spits and coworkers
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
12443
Promoter:
LTR
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Ellen Rothenberg
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Constitutive expression of PU.1 in fetal hematopoietic progenitors blocks T cell development at the pro-T cell stage. Anderson et al (Immunity. 2002 Feb;16(2):285-96. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34835" in your Materials and Methods section.