Vector backbonepTK380 (tetracycline inducible)
Backbone manufacturerDr. Tal Kafri
Growth in Bacteria
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesM. musculus (mouse)
Entrez GeneSnai1 (a.k.a. Sna, Sna1, Snail, Snail1)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Snail cDNA was PCR amplified from IMAGE clone and cloned into BamHI-XbaI sites of pTK380.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTK-Snail was a gift from Bob Weinberg (Addgene plasmid # 36976)
For your References section:Slug and Sox9 cooperatively determine the mammary stem cell state. Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van Oudenaarden A, Weinberg RA. Cell. 2012 Mar 2;148(5):1015-28. 10.1016/j.cell.2012.02.008 PubMed 22385965
Map generated by Addgene from plasmid data.