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pTK-Snail
(Plasmid #36976)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pTK380 (tetracycline inducible)
  • Backbone manufacturer
    Dr. Tal Kafri
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Snail
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Snai1 (a.k.a. RP23-118A2.5, AI194338, Sna, Sna1, Snail, Snail1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Snail cDNA was PCR amplified from IMAGE clone and cloned into BamHI-XbaI sites of pTK380.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTK-Snail was a gift from Bob Weinberg (Addgene plasmid # 36976)
  • For your References section:

    Slug and Sox9 cooperatively determine the mammary stem cell state. Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van Oudenaarden A, Weinberg RA. Cell. 2012 Mar 2;148(5):1015-28. 10.1016/j.cell.2012.02.008 PubMed 22385965