|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36977||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepTK380 (tetracycline inducible)
Backbone manufacturerDr. Tal Kafri
Growth in Bacteria
Insert Size (bp)1284
Entrez GeneTwist1 (a.k.a. M-Twist, Pde, Ska10, Ska<m10Jus>, Twist, bHLHa38, pdt)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Twist1 cDNA was cut from pBP-Twist (Addgene plasmid 1783) and cloned into BamHI-XhoI (blunt) sites of pTK380.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTK-Twist was a gift from Bob Weinberg (Addgene plasmid # 36977 ; http://n2t.net/addgene:36977 ; RRID:Addgene_36977)
For your References section:Slug and Sox9 cooperatively determine the mammary stem cell state. Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van Oudenaarden A, Weinberg RA. Cell. 2012 Mar 2;148(5):1015-28. 10.1016/j.cell.2012.02.008 PubMed 22385965