Addgene - pTK-Twist Plasmid Data

Plasmid 36977: pTK-Twist

Gene/insert name:
Twist
Insert size:
1284
Entrez Gene:
Twist1 (AA960487, M-Twist, Pde, Ska10, Ska<m10Jus>, Twist, bHLHa38, pdt)
Vector backbone:
pTK380 (tetracycline inducible)
(Search Vector Database)
Backbone manufacturer:
Dr. Tal Kafri
Vector type:
Lentiviral
Cloning site 5':
BamHI
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI
Site destroyed during cloning:
Yes
5' sequencing primer:
unknown List of Sequencing Primers
3' sequencing primer:
unknown
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Comments:

Twist1 cDNA was cut from pBP-Twist (Addgene plasmid 1783) and cloned into BamHI-XhoI (blunt) sites of pTK380.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Slug and Sox9 cooperatively determine the mammary stem cell state. Guo et al (Cell. 2012 Mar 2;148(5):1015-28. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36977" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

40646: pLKO.1-sh-mSOX9-5