Addgene - pBABE-puro-mTwist Plasmid Data

Plasmid 1783: pBABE-puro-mTwist

Gene/insert name:
Twist
Insert size:
1284
Species:
M. musculus (mouse)
GenBank ID:
M63649
Entrez Gene:
Twist1 (AA960487, M-Twist, Pde, Ska10, Ska<m10Jus>, Twist, bHLHa38, pdt)
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
SnaBI
Site destroyed during cloning:
Yes
Cloning site 3':
SnaBI
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
F. Perrin-Schmitt
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Yang et al (Cell 2004 Jun 25;117(7):927-39. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1783" in your Materials and Methods section.