Modified Shipping Schedule: Addgene will be closed November 26th & 27th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of Nov 23 - 27. If you have any questions, please contact us at

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at Learn more

(Plasmid #1783)

Add to Cart
Available to Academic and Nonprofits Only


  • Vector backbone
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.


  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Twist1 (a.k.a. AA960487, M-Twist, Pde, Ska10, Ska<m10Jus>, Twist, bHLHa38, pdt)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    F. Perrin-Schmitt
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-puro-mTwist was a gift from Bob Weinberg (Addgene plasmid # 1783)
  • For your References section:

    Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Yang J, Mani SA, Donaher JL, Ramaswamy S, Itzykson RA, Come C, Savagner P, Gitelman I, Richardson A, Weinberg RA. Cell 2004 Jun 25;117(7):927-39. 10.1016/j.cell.2004.06.006 PubMed 15210113