Addgene - Twist-siRNA7 Plasmid Data

Plasmid 8457: Twist-siRNA7

Gene/insert name:
siTwist
Alt name:
siRNA Twist
Alt name:
mTwist
Alt name:
Twist
Species:
M. musculus (mouse)
GenBank ID:
M63649
Entrez Gene:
Twist1 (AA960487, M-Twist, Pde, Ska10, Ska<m10Jus>, Twist, bHLHa38, pdt)
Vector backbone:
pSP-108
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
9439
Cloning site 5':
BstBI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
Empty pSP108 vector can be obtained from Dr. Eric Fearson, Univ of Michigan
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Twist-siRNA7-targetting sequence is AGCGGGTCATGGCTAACGTGC

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Yang et al (Cell 2004 Jun 25;117(7):927-39. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 8457" in your Materials and Methods section.