Addgene - pWZL Blast Twist ER Plasmid Data

Plasmid 18799: pWZL Blast Twist ER

Gene/insert name:
twist
Species:
M. musculus (mouse)
Entrez Gene:
Twist1 (AA960487, M-Twist, Pde, Ska10, Ska<m10Jus>, Twist, bHLHa38, pdt)
Fusion protein or tag:
ER
Terminal:
C terminal on insert
Vector backbone:
pWZL-Blast
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Blasticidin
Sequence: View sequences (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

pWZL-Blast-Twist-ER was
generated by replacing the MYC cDNA of pWZL-Blast-DN-MycER
(Littlewood et al., 1995; Watnick et al., 2003) with the mTwist cDNA PCR amplified from pBp-mTwist.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The epithelial-mesenchymal transition generates cells with properties of stem cells. Mani et al (Cell. 2008 May 16. 133(4):704-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18799" in your Materials and Methods section.