Addgene - pWZL Blast Snail ER Plasmid Data

Plasmid 18798: pWZL Blast Snail ER

Gene/insert name:
snail
Species:
H. sapiens (human)
Entrez Gene:
SNAI1 (SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
Fusion protein or tag:
ER
Terminal:
C terminal on insert
Mutation:
G525R mutation in the ER. The presence of the G525R point mutation in the cloned ER sequence abrogates its binding to 17β-estradiol while keeping its full sensitivity to the synthetic ligand 4'hydroxytamoxifen (4OHT).
Vector backbone:
pWZL-Blast
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
Yes
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Blasticidin
Sequence: View sequences (3)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

pWZL-Blast-Snail-ER was
generated by replacing the MYC cDNA of pWZL-Blast-DN-MycER
(Littlewood et al., 1995; Watnick et al., 2003) with the hSnail cDNA PCR amplified from pBp-hSnail.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The epithelial-mesenchymal transition generates cells with properties of stem cells. Mani et al (Cell. 2008 May 16. 133(4):704-15. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18798" in your Materials and Methods section.