pWZL Blast Snail ER
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18798||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5100
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
SpeciesH. sapiens (human)
MutationG525R mutation in the ER. The presence of the G525R point mutation in the cloned ER sequence abrogates its binding to 17β-estradiol while keeping its full sensitivity to the synthetic ligand 4'hydroxytamoxifen (4OHT).
Entrez GeneSNAI1 (a.k.a. SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1)
/ Fusion Protein
- ER hormone binding domain (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer IRES-R (5'-GCATTCCTTTGGCGAGAG-3') (Common Sequencing Primers)
generated by replacing the MYC cDNA of pWZL-Blast-DN-MycER
(Littlewood et al., 1995; Watnick et al., 2003) with the hSnail cDNA PCR amplified from pBp-hSnail.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pWZL Blast Snail ER was a gift from Bob Weinberg (Addgene plasmid # 18798 ; http://n2t.net/addgene:18798 ; RRID:Addgene_18798)
For your References section:The epithelial-mesenchymal transition generates cells with properties of stem cells. Mani SA, Guo W, Liao MJ, Eaton EN, Ayyanan A, Zhou AY, Brooks M, Reinhard F, Zhang CC, Shipitsin M, Campbell LL, Polyak K, Brisken C, Yang J, Weinberg RA. Cell. 2008 May 16. 133(4):704-15. 10.1016/j.cell.2008.03.027 PubMed 18485877