Addgene - pWZL Blast GFP Plasmid Data

Plasmid 12269: pWZL Blast GFP

Gene/insert name:
EGFP
Insert size:
800
Vector backbone:
pWZL Blast
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

An 800bp GFP cDNA fragment was cloned into the EcoRI site of the pWZL-blast vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Orimo et al (Cell. 2005 May 6. 121(3):335-48. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12269" in your Materials and Methods section.