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pWZL Blast myc
(Plasmid #10674)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10674 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pWZL Blast
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-myc
  • Alt name
    myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL Blast myc was a gift from William Hahn (Addgene plasmid # 10674 ; http://n2t.net/addgene:10674 ; RRID:Addgene_10674)
  • For your References section:

    Transformation of human and murine fibroblasts without viral oncoproteins. Boehm JS, Hession MT, Bulmer SE, Hahn WC. Mol Cell Biol. 2005 Aug . 25(15):6464-74. 10.1128/MCB.25.15.6464-6474.2005 PubMed 16024784