LAP2-beta-actin in modified pEGFP
(Plasmid #34839)

• Depositing Lab: Alice Ting
• Publication: Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9.

Backbone

• Vector backbone: Clontech
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LAP2-beta-Actin
• Species: H. sapiens (human)
• Insert Size (bp): 1218
• Entrez Gene: ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV.F
• 3′ sequencing primer: SV40.R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Beta-actin in Clontech was a gift from Mike Davidson at FSU.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry