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Bacterial strain 34929: BL21∆serB
  • None

  • None
    (Search Vector Database)

  • None

  • BL21

  • 37

  • Unknown

  • Jesse Rinehart

  • Dieter Soll

  • UBMTA

Comments: 

To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21. Markerless gene deletions were carried out using a λ-red and FLP recombinase-based gene knockout strategy.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

Article: Expanding the genetic code of Escherichia coli with phosphoserine. Park et al (Science. 2011 Aug 26;333(6046):1151-4. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34929" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only