BL21∆serB
(Bacterial strain #34929)

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Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    None
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21
  • Copy number
    Unknown

Sequence Information

  • Depositor Sequences
    None.
  • Addgene Sequences

Full plasmid sequence is available only if provided by the depositing laboratory.

Resource Information

  • Terms and Licenses

Depositor Comments

To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which catalyzes the last step in serine biosynthesis, was deleted from Escherichia coli strain BL21. Markerless gene deletions were carried out using a λ-red and FLP recombinase-based gene knockout strategy.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BL21∆serB was a gift from Jesse Rinehart & Dieter Soll (Addgene plasmid # 34929)
  • For your References section:

    Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ, Rinehart J, Soll D. Science. 2011 Aug 26;333(6046):1151-4. 10.1126/science.1207203 PubMed 21868676