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pCAT112TAG-SepT
(Plasmid #34624)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34624 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYC184
  • Backbone size w/o insert (bp) 3200
  • Modifications to backbone
    The gene encoding chloramphenicol acetyltransferae (CAT) was modified to introduce an amber stop codon at position Asp112 (pCAT112TAG).
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plate separately on Tet and Chlor plates.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tRNA^Sep
  • Insert Size (bp)
    75
  • Promoter lpp

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13_pUC-fwd
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAT112TAG-SepT was a gift from Jesse Rinehart & Dieter Söll (Addgene plasmid # 34624 ; http://n2t.net/addgene:34624 ; RRID:Addgene_34624)
  • For your References section:

    Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ, Rinehart J, Soll D. Science. 2011 Aug 26;333(6046):1151-4. 10.1126/science.1207203 PubMed 21868676