pCAT112TAG-SepT
(Plasmid #34624)

• Depositing Labs: Jesse Rinehart, Dieter Söll
• Publication: Park et al Science. 2011 Aug 26;333(6046):1151-4.

Backbone

• Vector backbone: pACYC184
• Backbone size w/o insert (bp): 3200
• Modifications to backbone: The gene encoding chloramphenicol acetyltransferae (CAT) was modified to introduce an amber stop codon at position Asp112 (pCAT112TAG).
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Tetracycline, 25 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Plate separately on Tet and Chlor plates.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tRNA^Sep
• Insert Size (bp): 75
• Promoter: lpp

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13_pUC-fwd

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry