|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34624||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3200
Modifications to backboneThe gene encoding chloramphenicol acetyltransferae (CAT) was modified to introduce an amber stop codon at position Asp112 (pCAT112TAG).
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Tetracycline
Growth instructionsPlate separately on Tet and Chlor plates.
Copy numberHigh Copy
Insert Size (bp)75
- Promoter lpp
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer M13_pUC-fwd (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAT112TAG-SepT was a gift from Jesse Rinehart & Dieter Söll (Addgene plasmid # 34624 ; http://n2t.net/addgene:34624 ; RRID:Addgene_34624)
For your References section:Expanding the genetic code of Escherichia coli with phosphoserine. Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ, Rinehart J, Soll D. Science. 2011 Aug 26;333(6046):1151-4. 10.1126/science.1207203 PubMed 21868676