To construct plasmid pSepT, the full-length gene encoding tRNA^Sep
was constructed from overlapping oligonucleotides and ligated immediately downstream of the lpp promoter in pTECH (Bunjun et al., 2000) using EcoRI and BamHI restriction sites.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
34625" in your Materials and Methods section.