|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34983||Plasmid sent as bacteria in agar stab||1||$65||Add to Cart|
Modifications to backbonePTEF–cpPDZ–mCherry–TCYC1 cassette inserted into YIPlac211
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
- Promoter TEF
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDS282 was a gift from Michael Glotzer (Addgene plasmid # 34983)
For your References section:TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. 10.1038/nmeth.1904 PubMed 22388287
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.