Addgene - pDS282 Plasmid Data

Plasmid 34983: pDS282

Gene/insert name:
cpPDZ–mCherry
Species:
S. cerevisiae (budding yeast)
Vector backbone:
YIPlac211
(Search Vector Database)
Vector type:
Yeast Expression
Modifications to Backbone:
PTEF–cpPDZ–mCherry–TCYC1 cassette inserted into YIPlac211
Promoter:
TEF
Cloning site 5':
SacI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
M13 fwd List of Sequencing Primers
3' sequencing primer:
M13 rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
URA3
Person or lab that originally
cloned the gene/insert:
Sequences from pGREG plasmids (EUROSCARF). cpPDZ CDS from Shohei Koide, University of Chicago
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Genbank sequence (application/octet-stream)
Principal Investigator:
Michael Glotzer
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland et al (Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 34983" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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