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pDS282
(Plasmid #34983)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34983 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    YIPlac211
  • Modifications to backbone
    PTEF–cpPDZ–mCherry–TCYC1 cassette inserted into YIPlac211
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cpPDZ–mCherry
  • Species
    S. cerevisiae (budding yeast)
  • Promoter TEF

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer M13 fwd
  • 3′ sequencing primer M13 rev
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sequences from pGREG plasmids (EUROSCARF). cpPDZ CDS from Shohei Koide, University of Chicago

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDS282 was a gift from Michael Glotzer (Addgene plasmid # 34983 ; http://n2t.net/addgene:34983 ; RRID:Addgene_34983)
  • For your References section:

    TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. 10.1038/nmeth.1904 PubMed 22388287