Addgene - pDEST14-SpyCatcher Plasmid Data

Plasmid 35044: pDEST14-SpyCatcher

Gene/insert name:
SpyCatcher
Alt name:
CnaB2114
Insert size:
420
Species:
Streptococcus pyogenes
Fusion protein or tag:
His6
Terminal:
N terminal on backbone
Mutation:
Deletion of C-terminal till I113, and point mutations I34E and M69Y
Vector backbone:
pDEST14
(Search Vector Database)
Vector type:
Bacterial Expression
Cloning method:
Gateway Cloning
5' sequencing primer:
T7F List of Sequencing Primers
3' sequencing primer:
T7R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Mark Howarth
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35044" in your Materials and Methods section.