Plasmid 35045: pDEST14-SpyCatcher EQ
  • SpyCatcher EQ

  • 420

  • Streptococcus pyogenes

  • His6

  • N terminal on backbone

  • Deletion of C-terminal till I113, and point mutations I34E and M69Y, and an inactivating point mutations of E77Q

  • pDEST14
    (Search Vector Database)

  • Bacterial Expression

  • Gateway Cloning

  • T7F List of Sequencing Primers

  • T7R

  • Ampicillin

  • XL1 Blue

  • 37

  • High Copy

  • View sequences (2)
  • Mark Howarth


Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35045" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only