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pDEST14-SpyCatcher EQ
(Plasmid #35045)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    SpyCatcher EQ
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    420
  • Mutation
    Deletion of C-terminal till I113, and point mutations I34E and M69Y, and an inactivating point mutations of E77Q
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST14-SpyCatcher EQ was a gift from Mark Howarth (Addgene plasmid # 35045)
  • For your References section:

    Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. 10.1073/pnas.1115485109 PubMed 22366317