Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35045||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Gene/Insert nameSpyCatcher EQ
Insert Size (bp)420
MutationDeletion of C-terminal till I113, and point mutations I34E and M69Y, and an inactivating point mutations of E77Q
/ Fusion Protein
- His6 (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDEST14-SpyCatcher EQ was a gift from Mark Howarth (Addgene plasmid # 35045 ; http://n2t.net/addgene:35045 ; RRID:Addgene_35045)
For your References section:Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. 10.1073/pnas.1115485109 PubMed 22366317