Addgene - pUC-mCA1-emptyMCS Plasmid Data

Plasmid 35095: pUC-mCA1-emptyMCS

Gene/insert name:
None
Vector backbone:
pUC
(Search Vector Database)
Vector type:
Mouse Targeting
Backbone size (bp):
2654
Modifications to Backbone:
mCA1 enhancer (243-2721) mCA1 colonic promoter (2967-13565) Multi cloning site (13566-13607) poly A signals (13608-14098)
Promoter:
mouse carbonic anhydrase 1
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL10 Gold
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
James Fleet
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestine. Xue et al (Mol Cancer Res. 2010 Aug;8(8):1095-104. Epub 2010 Jul 27. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35095" in your Materials and Methods section.