pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
(Plasmid #35152)

• Depositing Labs: Jay Keasling, Taek Soon Lee
• Publication: Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.

Backbone

• Vector backbone: pBbA5c
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
• Species: S. cerevisiae (budding yeast)
• Mutation: R364S in Mevalonate Kinase
• Promoter: LacUV5, pTrc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1, BglII (not destroyed)
• 3′ cloning site: BamH1, Xho1 (not destroyed)
• 5′ sequencing primer: gatcgtttaggcacccca
• 3′ sequencing primer: ATTAATAAGATGATC

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the R364 in Mevalonate Kinase (MK) may be susceptible to mutations. Although the original plasmid encoded R364C, Addgene's stock contains R364S. This mutation is not expected to impact overall function, however the exact effects are unknown.