Addgene - pLDPuro-MmnSR100N Plasmid Data

Plasmid 35171: pLDPuro-MmnSR100N

Gene/insert name:
Srrm4
Alt name:
nSR100
Insert size:
1800
Species:
M. musculus (mouse)
Entrez Gene:
Srrm4 (1500001A10Rik, B230202K19Rik, mKIAA1853, nSR100)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pLDPuro-TcVAPrtTA
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
10460
Cloning method:
Gateway Cloning
5' sequencing primer:
TRE-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
High or low copy:
High Copy
Sequence: View sequences (2)
Supplemental document:
pLD_puro_TcVAP_rtTA (application/pdf)
Principal Investigator:
Benjamin Blencowe
Terms and Licenses:
MTA

Comments:

Addgene sequencing found an additional N-term FLAG tag not present in the author's sequence--this does not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cross-regulation between an alternative splicing activator and a transcription repressor controls neurogenesis. Raj et al (Mol Cell. 2011 Sep 2;43(5):843-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35171" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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