hSyn1-sypHy-RGECO
(Plasmid #84078)

• Purpose: Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis
• Depositing Lab: Juan Burrone
• Publication: Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:21. doi: 10.3389/fnsyn.2016.00021. eCollection 2016.

Backbone

• Vector backbone: pEGFP-N2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3903
• Total vector size (bp): 6846
• Modifications to backbone: CMV promoter replaced with hSyn1 promoter, EGFP removed.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sypHy-RGECO
• Alt name: Rat Synaptophysin-pHluorin and R-GECO1
• Species: R. norvegicus (rat), Synthetic
• Insert Size (bp): 2943
• Promoter: hSyn1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ACTCAGCGCTGCCTCAGTC
• 3′ sequencing primer: TTATGTTTCAGGTTCAGGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SypHy was subcloned from CMV-SypHy A4 (Addgene 24478) and R-GECO1 from CMV-R-GECO1 (Addgene 32444)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry