Addgene - MIGS1.1 (JW493) Plasmid Data

Plasmid 35245: MIGS1.1 (JW493)

Gene/insert name:
None
Vector backbone:
pGreenII
(Search Vector Database)
Vector type:
plant expression
Backbone size (bp):
7647
Modifications to Backbone:
miR173 target site add between 35S promoter and attR1 site
Promoter:
CaMV 35S
Bacterial resistance(s)
Spectinomycin
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
1) Before recombination Chloramphenicol might be used for bacteria selection (resistance lost after recombination). 2) E. coli DB3.1 strain (or some other ccdB survival strain) should be used for the amplification of the plasmid due to the presence of the lethal ccdB gene. After recombination, any common strain, such as DH5alpha is recommended. 3) Due to pGreen backbone characteristics, the helper plasmid pSOUP is required for replication of MIGS vector in Agrobacterium. PSOUP can be requested directly at http://www.pgreen.ac.uk/.
High or low copy:
High Copy
Selectable markers:
Basta
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Detlef Weigel
Terms and Licenses:
MTA

Comments:

MIGS vectors are based on pGREEN series:

Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.

http://www.pgreen.ac.uk/

obtained from John Innes Centre, UK

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: MIGS: MiRNA Induced Gene Silencing. de et al (Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35245" in your Materials and Methods section.