1
Plasmid 35247: MIGS2.1 (FF570)
  • None

  • pGreenII
    (Search Vector Database)

  • Plant Expression

  • 9298

  • 1) miR173 target site add between 35S promoter and attR1 site. 2) Addition of an expression cassette with the miR173 precursor placed behind the UBQ11 constitutive promoter

  • CaMV 35S

  • Spectinomycin and Chloramphenicol

  • ccdB Survival

  • 37

  • 1) Before recombination Chloramphenicol might be used for bacteria selection (resistance lost after recombination). 2) E. coli DB3.1 strain (or some other ccdB survival strain) should be used for the amplification of the plasmid due to the presence of the lethal ccdB gene. After recombination, any common strain, such as DH5alpha is recommended. 3) Due to pGreen backbone characteristics, the helper plasmid pSOUP is required for replication of MIGS vector in Agrobacterium. PSOUP can be requested directly at http://www.pgreen.ac.uk/.

  • High Copy

  • Basta

  • View sequences (5)
  • View map

  • Detlef Weigel

  • UBMTA

Comments: 

MIGS vectors are based on pGREEN series:

Hellens, R.P., Edwards, E.A., Leyland, N.R., Bean, S. and Mullineaux, P.M. (2000)
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant
transformation. Plant Mol. Biol., 42, 819-832.

http://www.pgreen.ac.uk/

obtained from John Innes Centre, UK

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: MIGS: MiRNA Induced Gene Silencing. de et al (Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35247" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only