Addgene - pGEX 4t1 RARalpha (1-187) Plasmid Data

Plasmid 35558: pGEX 4t1 RARalpha (1-187)

Gene/insert name:
RARalpha
Insert size:
561
Species:
H. sapiens (human)
Entrez Gene:
RARA (NR1B1, RAR)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Mutation:
aa 1-187
Vector backbone:
pGEX 4T1
(Search Vector Database)
Vector type:
Bacterial Expression
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
NA List of Sequencing Primers
3' sequencing primer:
NA
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jonathan Kurie
Principal Investigator:
Harish Srinivas
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: c-Jun N-terminal kinase contributes to aberrant retinoid signaling in lung cancer cells by phosphorylating and inducing proteasomal degradation of retinoic acid receptor alpha. Srinivas et al (Mol Cell Biol. 2005 Feb;25(3):1054-69. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35558" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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