pGFPns-reporter
(Plasmid #35626)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    NA
  • Backbone size (bp) 6290
  • Vector type
    Mammalian Expression ; Reporter
  • Promoter CMV
  • Selectable markers
    Neomycin
  • Tag / Fusion Protein
    • GFPns (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Resource Information

Depositor Comments

Meant to be used in combination with pAAV-Ptet-RFP-shR-rtTA (Addgene #35625) to test the efficacy of shRNAs.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFPns-reporter was a gift from Howard Gu (Addgene plasmid # 35626)
  • For your References section:

    Fluorescence-based evaluation of shRNA efficacy. Naughton BJ, Han DD, Gu HH. Anal Biochem. 2011 Oct 1;417(1):162-4. Epub 2011 Jun 13. 10.1016/j.ab.2011.06.008 PubMed 21726522