Addgene - pLenti6-BMPR1A(Q233D)-V5 Plasmid Data

Plasmid 35638: pLenti6-BMPR1A(Q233D)-V5

Gene/insert name:
BMP Receptor Type1A Q233D
Alt name:
BMPR1A
Insert size:
1602
Species:
H. sapiens (human)
Entrez Gene:
BMPR1A (10q23del, ACVRLK3, ALK3, CD292, SKR5)
Fusion protein or tag:
V5
Terminal:
C terminal on insert
Mutation:
Glutamine 233 to Aspartate
Vector backbone:
pLenti6
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
10000
Promoter:
CMV
Cloning method:
Gateway Cloning
5' sequencing primer:
attL1 List of Sequencing Primers
3' sequencing primer:
attL2
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (3)
Principal Investigator:
Daniel Haber
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers. Singh et al (Cell. 2012 Feb 17;148(4):639-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 35638" in your Materials and Methods section.