pAAV TBG FFluc miR122sponge
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35657||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepAAV TBG FFluc
- Backbone size w/o insert (bp) 5915
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemiR-122 sponge
Insert Size (bp)156
- Promoter TBG
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR V (destroyed during cloning)
- 3′ cloning site Apa I (not destroyed)
- 5′ sequencing primer tgttgttttggagcacggaaaga (Common Sequencing Primers)
Please note that there is a 4bp deletion in Addgene's quality control sequence when compared to depositor's reference sequence. This deletion will not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV TBG FFluc miR122sponge was a gift from Phillip Zamore (Addgene plasmid # 35657 ; http://n2t.net/addgene:35657 ; RRID:Addgene_35657)