sc AAV miR26a eGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21894||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3952
Vector typeMammalian Expression, AAV ; sc
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEF1 alpha -miR26a GFP p(A)
Insert Size (bp)2099
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Spe I (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Article Citing this Plasmid
miR-26a sequence is 5'-TTCAAGTAATCCAGGATAGGC
Addgene sequencing results confirm the presence of both FseI sites flanking miR-26a.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:sc AAV miR26a eGFP was a gift from Joshua Mendell (Addgene plasmid # 21894 ; http://n2t.net/addgene:21894 ; RRID:Addgene_21894)
For your References section:Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Mendell JR, Mendell JT. Cell. 2009 Jun 12. 137(6):1005-17. 10.1016/j.cell.2009.04.021 PubMed 19524505