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pRS424GFP-FYVE(EEA1)
(Plasmid #36096)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36096 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS424
  • Modifications to backbone
    To create a yeast GFP expression vector (pGOGFP), the PRC1 promoter and GFP coding sequences were cloned into the BamHI site of pRS424.
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FYVE(EEA1) domain
  • Species
    H. sapiens (human)
  • Entrez Gene
    EEA1 (a.k.a. MST105, MSTP105, ZFYVE2)
  • Promoter PRC1
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unkown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS424GFP-FYVE(EEA1) was a gift from Scott Emr (Addgene plasmid # 36096 ; http://n2t.net/addgene:36096 ; RRID:Addgene_36096)
  • For your References section:

    Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Burd CG, Emr SD. Mol Cell. 1998 Jul;2(1):157-62. 10.1016/S1097-2765(00)80125-2 PubMed 9702203