TagRFP-T-EEA1
(Plasmid #42635)

• Depositing Lab: Silvia Corvera
• Publication: Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):E471-80. Epub 2012 Jan 30.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 4236
• Modifications to backbone: TagRFP-T from Roger Tsien was used to replace the GFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EEA1
• Alt name: Early Endosome Antigen 1
• Species: H. sapiens (human)
• Insert Size (bp): 4236
• Mutation: none
• GenBank ID: NM_003566
• Entrez Gene: EEA1 (a.k.a. MST105, MSTP105, ZFYVE2)
• Promoter: CMV
• Tag / Fusion Protein:
  • TagRFP-T (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (destroyed during cloning)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry