Addgene - YFP Gamma 3 Plasmid Data

Plasmid 36103: YFP Gamma 3

Gene/insert name:
G protein subunit Gamma 3
Alt name:
Gng3
Insert size:
228
Species:
M. musculus (mouse)
GenBank ID:
BC029680.1
Entrez Gene:
Gng3 ()
Fusion protein or tag:
YFP
Terminal:
N terminal on insert
Vector backbone:
pDEST12.2
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
7261
Promoter:
CMV
Cloning method:
Gateway Cloning
5' sequencing primer:
Sp6 primer List of Sequencing Primers
3' sequencing primer:
T7 primer
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Narasimhan Gautam
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A family of G protein betagamma subunits translocate reversibly from the plasma membrane to endomembranes on receptor activation. Saini et al (J Biol Chem. 2007 Aug 17;282(33):24099-108. Epub 2007 Jun 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36103" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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