Addgene - YFP Gamma 12 Plasmid Data

Plasmid 36109: YFP Gamma 12

Gene/insert name:
G protein subunit Gamma 12
Alt name:
Gng12
Insert size:
219
Species:
M. musculus (mouse)
GenBank ID:
NM_025278
Entrez Gene:
Gng12 (2010305F15Rik, AA536815, AI115529, AI314170, AI842738)
Fusion protein or tag:
YFP
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3.1
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5428
Promoter:
CMV
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
EcoR1
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH reverse primer
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Narasimhan Gautam
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A family of G protein betagamma subunits translocate reversibly from the plasma membrane to endomembranes on receptor activation. Saini et al (J Biol Chem. 2007 Aug 17;282(33):24099-108. Epub 2007 Jun 20. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36109" in your Materials and Methods section.

Price: $65.00

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