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BasiginCD4d3+4-Comp-His-Stop
(Plasmid #36147)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36147 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Basigin
  • Alt name
    BSG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    970
  • Entrez Gene
    BSG (a.k.a. 5F7, CD147, EMMPRIN, EMPRIN, OK, SLC7A11, TCSF)
  • Promoter CMV
  • Tags / Fusion Proteins
    • ratCD4d3+4 (C terminal on insert)
    • pentamerization domain of rat cartilaginous oligomeric matrix protein (COMP) (C terminal on insert)
    • His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BasiginCD4d3+4-Comp-His-Stop was a gift from Gavin Wright (Addgene plasmid # 36147 ; http://n2t.net/addgene:36147 ; RRID:Addgene_36147)
  • For your References section:

    Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, Uchikawa M, Mboup S, Ndir O, Kwiatkowski DP, Duraisingh MT, Rayner JC, Wright GJ. Nature. 2011 Nov 9;480(7378):534-7. doi: 10.1038/nature10606. 10.1038/nature10606 PubMed 22080952