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CD200RCD4d3+4-bio-linker-His
(Plasmid #36153)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36153 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7340
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD200R
  • Alt name
    OX-2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    714
  • Entrez Gene
    Cd200r1 (a.k.a. Mox2r)
  • Promoter CMV
  • Tags / Fusion Proteins
    • ratCD4d3+4 (C terminal on backbone)
    • biotinylation peptide (C terminal on backbone)
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD200RCD4d3+4-bio-linker-His was a gift from Gavin Wright (Addgene plasmid # 36153 ; http://n2t.net/addgene:36153 ; RRID:Addgene_36153)
  • For your References section:

    Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487