Addgene - CD200RCD4d3+4-bio-linker-His Plasmid Data

Plasmid 36153: CD200RCD4d3+4-bio-linker-His

Gene/insert name:
CD200R
Alt name:
OX-2
Insert size:
714
Species:
R. norvegicus (rat)
Entrez Gene:
Cd200r1 (Mox2r)
Fusion protein or tag:
ratCD4d3+4
Terminal:
C terminal on backbone
Fusion protein or tag:
biotinylation peptide
Terminal:
C terminal on backbone
Fusion protein or tag:
His tag
Terminal:
C terminal on backbone
Vector backbone:
pTT3
(Search Vector Database)
Backbone manufacturer:
Yves Durocher, NRC
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6000
Promoter:
CMV promoter
Promoter:
CMV
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
AscI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Gavin Wright
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Large-scale screening for novel low-affinity extracellular protein interactions. Bushell et al (Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 36153" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

32408: secretedBirA-8his

32405: CD200Cd4d3+4-blac