pTriExRhoA2G
(Plasmid #40176)

• Depositing Lab: Olivier Pertz
• Publication: Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135.

Backbone

• Vector backbone: pTriEx4
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 7500
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RhoA Biosensor
• Species: Synthetic
• Insert Size (bp): 2570
• Promoter: CMV
• Tag / Fusion Protein:
  • His-Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: ctggttattgtgctgtctc
• 3′ sequencing primer: tcagtggtatttgtga

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry